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Generation and repair of AID-initiated DNA lesions in B lymphocytes
null
《医学前沿(英文)》 2014年 第8卷 第2期 页码 201-216 doi: 10.1007/s11684-014-0324-4
Activation-induced deaminase (AID) initiates the secondary antibody diversification process in B lymphocytes. In mammalian B cells, this process includes somatic hypermutation (SHM) and class switch recombination (CSR), both of which require AID. AID induces U:G mismatch lesions in DNA that are subsequently converted into point mutations or DNA double stranded breaks during SHM/CSR. In a physiological context, AID targets immunoglobulin (Ig) loci to mediate SHM/CSR. However, recent studies reveal genome-wide access of AID to numerous non-Ig loci. Thus, AID poses a threat to the genome of B cells if AID-initiated DNA lesions cannot be properly repaired. In this review, we focus on the molecular mechanisms that regulate the specificity of AID targeting and the repair pathways responsible for processing AID-initiated DNA lesions.
关键词: class switch recombination somatic hypermutation activation-induced deaminase DNA repair genomic instability
The role of PARP1 in the DNA damage response and its application in tumor therapy
null
《医学前沿(英文)》 2012年 第6卷 第2期 页码 156-164 doi: 10.1007/s11684-012-0197-3
Single-strand break repair protein poly(ADP-ribose) polymerase 1 (PARP1) catalyzes the poly(ADP-ribosyl)ation of many key proteins in vivo and thus plays important roles in multiple DNA damage response pathways, rendering it a promising target in cancer therapy. The tumor-suppressor effects of PARP inhibitors have attracted significant interest for development of novel cancer therapies. However, recent evidence indicated that the underlying mechanism of PARP inhibitors in tumor therapy is more complex than previously expected. The present review will focus on recent progress on the role of PARP1 in the DNA damage response and PARP inhibitors in cancer therapy. The emerging resistance of BRCA-deficient tumors to PARP inhibitors is also briefly discussed from the perspective of DNA damage and repair. These recent research advances will inform the selection of patient populations who can benefit from the PARP inhibitor treatment and development of effective drug combination strategies.
关键词: PARP1 synthetic lethality PARP inhibitor DNA repair cancer NHEJ
Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 26-29 doi: 10.1007/s11684-009-0008-7
关键词: Ku80 DNA-PK(cs)-binding protein human ataxia telangiectasia mutated protein tumor cell lines radiosensitivity
Regulation and function of histone acetyltransferase MOF
null
《医学前沿(英文)》 2014年 第8卷 第1期 页码 79-83 doi: 10.1007/s11684-014-0314-6
The mammalian MOF (male absent on the first), a member of the MYST (MOZ, YBF2, SAS2, and Tip60) family of histone acetyltransferases (HATs), is the major enzyme that catalyzes the acetylation of histone H4 on lysine 16. Acetylation of K16 is a prevalent mark associated with chromatin decondensation. MOF has recently been shown to play an essential role in maintaining normal cell functions. In this study, we discuss the important roles of MOF in DNA damage repair, apoptosis, and tumorigenesis. We also analyze the role of MOF as a key regulator of the core transcriptional network of embryonic stem cells.
关键词: MOF histone acetyltransferase DNA damage repair tumorigenesis embryonic stem cells
Bile duct injury repair — earlier is not better
null
《医学前沿(英文)》 2015年 第9卷 第4期 页码 508-511 doi: 10.1007/s11684-015-0418-7
Bile duct injury is a common complication of cholecystectomy. The timing of bile duct injury repair remains controversial. A recent review conducted in France reported 39% complications and 64% failure after immediate repair in 194 patients compared with 14% complications and 8% failure after late repair in 133 patients. A national review of 139 consecutive early repairs conducted at five hepatopancreaticobiliary centers in Denmark reported 4% mortality, 36% morbidity, and 42 restrictures (30%) at a median follow-up of 102 months, and only 64 patients (46%) demonstrated uneventful short-term and long-term outcomes. Most patients with bile duct injury present with bile leak and sepsis; thus, early repair is not recommended. Percutaneous drainage of bile and endoscopic stenting are the mainstays of treatment of bile leak because they convert acute bile duct injury into a controlled external biliary fistula. The ensuing benign biliary stricture should be repaired by a biliary surgeon after a delay of 4–6 weeks once the external biliary fistula has closed.
关键词: bile duct injury cholecystectomy laparoscopic cholecystectomy
工程化DNA材料构建DNA活字系统实现可持续的数据存储 Article
巩子祎, 宋理富, 裴广胜, 董雨菲, 李炳志, 元英进
《工程(英文)》 2023年 第29卷 第10期 页码 130-136 doi: 10.1016/j.eng.2022.05.023
DNA分子作为一种具有潜力的数据存储绿色材料,具有密度高和保存期长的优势。然而,目前DNA数据存储的数据写入依赖于DNA从头合成,写入成本高昂,且产生有害物,限制了其实际应用。在本研究中,我们开发了一种DNA活字存储系统,该系统可以利用由细胞工厂预生产的DNA活字片段进行数据写入。在这个系统中,这些预先生成的DNA片段,在这里称为“DNA活字”,是可重复使用的基本数据单元。通过这些DNA活字的快速组装来实现数据写入,从而避免了昂贵且对环境有害的DNA化学合成过程。通过DNA活字片段的反复使用和生物组装,该系统在降低写入成本方面的潜力非常突出,为经济和可持续的DNA数据存储技术开辟了一条新颖路线。
关键词: 合成生物学 DNA信息存储 DNA活字存储系统 经济性DNA数据存储
Mobile platform for hydraulic turbine blade repair robot
GUI Zhongcheng, CHEN Qiang, SUN Zhenguo, ZHANG Wenzeng, LIU Kang
《机械工程前沿(英文)》 2008年 第3卷 第2期 页码 164-169 doi: 10.1007/s11465-008-0035-0
Expression status of GATA3 and mismatch repair proteins in upper tract urothelial carcinoma
Yue Wang, Jinxia Zhang, Yunfan Wang, Shufang Wang, Yu Zhang, Qi Miao, Fei Gao, Huiying He
《医学前沿(英文)》 2019年 第13卷 第6期 页码 730-740 doi: 10.1007/s11684-019-0687-7
关键词: upper tract urothelial carcinoma GATA binding protein 3 mismatch repair microsatellite instability prognosis
Functional role of ATM in the cellular response to DNA damage
Ming LIU, Wenxiang HU
《化学科学与工程前沿(英文)》 2011年 第5卷 第2期 页码 179-187 doi: 10.1007/s11705-009-0268-4
关键词: ataxia-telangiectasia mutated (ATM) cell cycle checkpoint DNA damage signalling transduction
Molecular simulation of the interaction mechanism between CodY protein and DNA in
Linchen Yuan, Hao Wu, Yue Zhao, Xiaoyu Qin, Yanni Li
《化学科学与工程前沿(英文)》 2019年 第13卷 第1期 页码 133-139 doi: 10.1007/s11705-018-1737-4
null
《医学前沿(英文)》 2015年 第9卷 第3期 页码 261-274 doi: 10.1007/s11684-015-0406-y
Environmental pollution is one of the main causes of human cancer. Exposures to environmental carcinogens result in genetic and epigenetic alterations which induce cell transformation. Epigenetic changes caused by environmental pollution play important roles in the development and progression of environmental pollution-related cancers. Studies on DNA methylation are among the earliest and most conducted epigenetic research linked to cancer. In this review, the roles of DNA methylation in carcinogenesis and their significance in clinical medicine were summarized, and the effects of environmental pollutants, particularly air pollutants, on DNA methylation were introduced. Furthermore, prospective applications of DNA methylation to environmental pollution detection and cancer prevention were discussed.
关键词: environmental pollution DNA methylation cancer biomarker diagnosis therapy prevention
Effects of DNA damage on oocyte meiotic maturation and early embryonic development
Shen YIN,Junyu MA,Wei SHEN
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 185-190 doi: 10.15302/J-FASE-2014035
表征不同DNA高阶结构的单分子分析方法 Review
刘泳麟, 边天元, 刘岩, 李治民, 裴羽丰, 宋杰
《工程(英文)》 2023年 第24卷 第5期 页码 277-292 doi: 10.1016/j.eng.2022.10.009
DNA不仅是生命遗传信息的载体,而且是一种高度可编程和自组装的纳米材料。不同的DNA结构与其生物学和化学功能有关。因此,了解各种DNA结构的物理和化学性质在生物学和纳米化学中具有重要意义。然而,群体分子实验忽略了溶液中DNA结构的异质性。单分子分析方法是观察单个分子的行为和探测自由能态的高异质性的有力工具。本文介绍了单分子检测和操纵等单分子分析方法,并讨论了这些方法如何用于测量单/双链DNA(ss/dsDNA)、DNA高阶结构和DNA纳米结构的分子性质。最后,将DNA纳米技术和单分子分析方法进行结合以了解DNA和其他生物物质、软物质的生物物理特性。
Yanhua GUAN, Ying GAO, Renjuan SUN, Moon C. WON, Zhi GE
《结构与土木工程前沿(英文)》 2017年 第11卷 第3期 页码 338-345 doi: 10.1007/s11709-017-0411-0
关键词: Calcium Sulfoaluminate (CSA) cement Type III cement coefficient of thermal expansion (CTE) shrinkage rapid repair
基于探针图的并行型图顶点着色DNA计算模型 Article
许进, 强小利, 张凯, 张成, 杨静
《工程(英文)》 2018年 第4卷 第1期 页码 61-77 doi: 10.1016/j.eng.2018.02.011
关键词: DNA计算 图顶点着色问题 聚合酶链反应(PCR)
标题 作者 时间 类型 操作
Correlativity study between expression of DNA double-strand break repair protein and radiosensitivity
Liang ZHUANG, Shiying YU, Xiaoyuan HUANG, Yang CAO, Huihua XIONG
期刊论文
Mobile platform for hydraulic turbine blade repair robot
GUI Zhongcheng, CHEN Qiang, SUN Zhenguo, ZHANG Wenzeng, LIU Kang
期刊论文
Expression status of GATA3 and mismatch repair proteins in upper tract urothelial carcinoma
Yue Wang, Jinxia Zhang, Yunfan Wang, Shufang Wang, Yu Zhang, Qi Miao, Fei Gao, Huiying He
期刊论文
Molecular simulation of the interaction mechanism between CodY protein and DNA in
Linchen Yuan, Hao Wu, Yue Zhao, Xiaoyu Qin, Yanni Li
期刊论文
Environmental pollution and DNA methylation: carcinogenesis, clinical significance, and practical applications
null
期刊论文
Effects of DNA damage on oocyte meiotic maturation and early embryonic development
Shen YIN,Junyu MA,Wei SHEN
期刊论文
Experimental study and field application of calcium sulfoaluminate cement for rapid repair of concrete
Yanhua GUAN, Ying GAO, Renjuan SUN, Moon C. WON, Zhi GE
期刊论文